Composite

Part:BBa_K1084124:Design

Designed by: Matsumura Naoya   Group: iGEM13_HokkaidoU_Japan   (2013-09-06)
Revision as of 13:51, 26 September 2013 by Barao (Talk | contribs)

pTet SD8 LacZα dT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This RBS has an sequence called an "enhancer". The enhancer is located upstream of the SD seqeuence. It enhances the binding of Ribosome and mRNA. Also, it is said that the strength of the RBS, depends on the length of SD sequence.


Source

coming soon

References

Translation initiation region sequence preferences in Escherichia coli Vladimir Vimberg, Age Tats, Maido Remm and Tanel Tenson