Coding
Part:BBa_K1184000:Design
Designed by: Eric Pederson Group: iGEM13_Carnegie_Mellon (2013-09-04)
KillerRed
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 151
Illegal BsaI.rc site found at 442
Design Notes
This sequence is not codon optimized for E. coli.
Sequence contains 14 rare proline codons (CCC) and a rare arginine codon (AGA).
Source
Engineered from anm2CP. Provided by the Bruchez lab at Carnegie Mellon