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Composite
luxR-ndh

Part:BBa_K1036000

Designed by: Shengquan Zeng, Zhaopeng Cheng   Group: iGEM13_XMU-China   (2013-08-27)
Revision as of 09:12, 3 September 2013 by Cproud (Talk | contribs) (→‎Protocol)

lux pL controlled luxR with lux pR controlled ndh (LVA-tag) coding for NADH dehydrogenase II

The ndh gene codes for NDH-2, an enzyme that generates

Usage and Biology

Protocol

Ndh with LVA tag is formed as a composite by the ligation of quorum sensing promoter luxR via digestion and ligation.

Experimental Data

SDS page, microfluid, and gel image of construction.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2544
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1101


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Categories
Parameters
n/alux pL controlled luxR with lux pR controlled ndh (LVA-tag) coding for NADH dehydrogenase II