Part:BBa_K1216001
Alkaline Phosphatase (phoA) from Citrobacter
The alkaline phosphatase is a periplasmic homodimeric hydrolase. Each monomer contains 429 amino acids.
Usage and Biology
Alkaline phosphatases are used as reporter enzymes in different assays such as Western Blotting and in situ hybridization. [1]
Alkaline phosphatases cleave phosphate groups from organic compounds by hydrolysis while retaining stereochemistry[2].
A good explanation of the mechanism can be found [http://chemwiki.ucdavis.edu/Organic_Chemistry/Organic_Chemistry_With_a_Biological_Emphasis/Chapter_10%3A_Phosphoryl_transfer_reactions/Section_10.3%3A_Hydrolysis_of__phosphates here].
Alkaline phosphatases, respectively their serum levels, are also related to several diseases e.g. metabolic myopathies and Paget Disease. [3]
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 340
Illegal NgoMIV site found at 787 - 1000COMPATIBLE WITH RFC[1000]
References
- Molecular Cell Biology, Fifth Edition, W.H. Freeman & Co., 2004.
- [http://chemwiki.ucdavis.edu/Organic_Chemistry/Organic_Chemistry_With_a_Biological_Emphasis/Chapter_10%3A_Phosphoryl_transfer_reactions/Section_10.3%3A_Hydrolysis_of__phosphates Section 10.3: Hydrolysis of phosphates]
- Adams & Victor's Principles Of Neurology, 7th edition, McGraw-Hill Professional, 2000.
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