Generator
Part:BBa_K1216001
Designed by: Parvathi Chandran Group: iGEM13_ETH_Zurich (2013-08-29)
Alkaline Phosphatase (phoA) from Citrobacter
The alkaline phosphatase is a periplasmic homodimeric hydrolase.
Usage and Biology
Alkaline phosphatases are used as reporter enzymes in different assays such as Western Blotting and in situ hybridization. [1]
Alkaline phosphatases, respectively their levels, are also related to several diseases e.g. metabolic myopathies and Paget Disease. [2]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 340
Illegal NgoMIV site found at 787 - 1000COMPATIBLE WITH RFC[1000]
References
1. Molecular Cell Biology, Fifth Edition, W.H. Freeman & Co., 2004. 2. Adams & Victor's Principles Of Neurology, 7th edition, McGraw-Hill Professional, 2000.
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