RBS

Part:BBa_K1084104:Design

Designed by: Ami Kawahata   Group: iGEM13_HokkaidoU_Japan   (2013-08-28)
Revision as of 14:51, 26 September 2013 by Barao (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

SD8


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This RBS has an sequence called an "enhancer". The enhancer is located upstream of the SD seqeuence. It enhances the binding of Ribosome and mRNA. Also, it is said that the strength of the RBS, depends on the length of SD sequence.


Source

Based on Vimberg et al. (2007), we construnted this part from synthetic oligos.

References

V. Vimberg, A. Tats, M. Remm T. Tenson, Translation initiation region sequence preferences in Escherichia coli (2007) BMC Molecular Biology