RBS
Part:BBa_K1084101:Design
Designed by: Ryotaro Nouda Group: iGEM13_HokkaidoU_Japan (2013-08-27)
SD2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This RBS has an sequence called an "enhancer". The enhancer is located upstream of the SD seqeuence. It enhances the binding of Ribosome and mRNA. Also, it is said that the strength of the RBS, depends on the length of SD sequence.
Source
coming soon
References
Translation initiation region sequence preferences in Escherichia coli Vladimir Vimberg, Age Tats, Maido Remm and Tanel Tenson