Reporter

Part:BBa_K1041000

Designed by: NRP UEA   Group: iGEM13_NRP-UEA-Norwich   (2013-08-08)
Revision as of 11:13, 24 August 2013 by Holusac (Talk | contribs) (→‎Pictures)

RFP Coding Device

Mutagenesis was used to add a Nde1 site at the start of the RFP coding region of the biobrick BBa_J04450. This enables a restriction digest with Nde1 to be performed allowing the RFP coding region to be excised from the plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    COMPATIBLE WITH RFC[1000]

Pictures

  • (Left) BBa_K1041000 and (right) BBa_J04450 visualized under non-UV lightbox

  • Both parts BBa K1041000 and BBa J04450 have colonies that appear red under natural light. The addition of a NdeI site has not effected the ability for the RFP gene to be transcribed.
[edit]
Categories
//classic/reporter/pret
Parameters
emissionRFP
excitation
tagNone