Coding

Part:BBa_K1033259

Designed by: Anders Edlund   Group: iGEM13_Uppsala   (2013-08-22)
Revision as of 14:54, 3 October 2013 by AndersE (Talk | contribs)

Toxin-antitoxin system from lactobacillus plantarum

This is a toxin-antitoxin system originating from plasmid p256 from lactobacillus plantarum strain NC7. It has been created without the natural promotor to allow for customisable strength of the system. The main use of the toxin-antitoxin system is to make the plasmid more stabile without having to rely on antibiotic resistance. The antitoxin has a shorter halflife then the antitoxin. If the cell were to lose the plasmid the toxin will remain longer in the cell and thereby kill it. It has been proven very efficient in previous studies (http://mic.sgmjournals.org/content/151/2/421.long).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 71
    Illegal SpeI site found at 447
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 71
    Illegal SpeI site found at 447
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 71
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 71
    Illegal SpeI site found at 447
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 71
    Illegal SpeI site found at 447
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None