Coding

Part:BBa_K1033001

Designed by: Karl Holdar   Group: iGEM13_Uppsala   (2013-08-23)
Revision as of 17:56, 4 October 2013 by HampusE (Talk | contribs)

4-coumarate ligase (4CL) with RBS

4-coumarate ligase (4CL) is an enzyme that catalyses the reaction from p-coumaric acid to 4-coumaroyl-coenzyme A (4-coumaryl-CoA). This enzyme is derived from the plant arabidhopsis thaliana, but exists in many other plants. [1]

In our project, we have been using it together with stilbene synthase, that produces resveratrol with the help of this enzyme.

Uppsala_4CL-STS.png

Applications With the help of this enzyme one can produce 4-coumaryl-CoA which is an important precursor in many metabolic pathways. For example, it can be used to produce resveratrol with stilbense synthase. [2]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1108
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1675
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1307


[edit]
Categories
//chassis/prokaryote/ecoli/nissle
//collections/probiotics/production
Parameters
None