Composite

Part:BBa_K1188000:Design

Designed by: Bill Heymann, Phil Jensen   Group: iGEM13_CU-Boulder   (2013-08-14)
Revision as of 02:24, 28 September 2013 by Immudzen (Talk | contribs)

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AmilCP screening part


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We needed a strong blue color to make it easy to distinguish this from a red carry through. We used a strong anderson promotor (J23100), standard RBS and hooked it to AmilCP. This part is also very useful for protein purification since the color is very strong and shows up well in an agarose gel.


Source

J23100, K592009 and B0034 came from the registry.

References