Coding
SoDA

Part:BBa_M36071:Design

Designed by: Alex Rosay, Justin Diep, Ben Blankenmeister   Group: Stanford BIOE44 - S11   (2012-12-07)
Revision as of 11:02, 7 December 2012 by Arosay (Talk | contribs) (→‎Design Notes)

K12 e.coli native Manganese Superoxide Dismutase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 346
    Illegal PstI site found at 283
    Illegal PstI site found at 593
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 346
    Illegal PstI site found at 283
    Illegal PstI site found at 593
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 346
    Illegal XhoI site found at 43
    Illegal XhoI site found at 127
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 346
    Illegal PstI site found at 283
    Illegal PstI site found at 593
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 346
    Illegal PstI site found at 283
    Illegal PstI site found at 593
    Illegal NgoMIV site found at 542
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This DNA sequence is converted from the amino acid sequence of Mn-SOD protein using the online converter from In-Silico. We optimized our sequence in DNA 2.0's Gene Designer by increasing GC content and minimizing repeats. We accomplished this by altering basepairs without altering amino acids.

Source

This sequence is derived from the amino acid sequence of Mn-SOD protein found from Durfee et. al at the University of Wisconsin which also provided the entire genomic sequence of the E. coli (strain: k-12, substrain: DH10B) genome.

References