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Part:BBa_K902066
pRha-B0034-K082003
Encodes for the pRha, RBS site and a GFP with an LVA tag. This circuit is used to characterize expression of the rhamnose promoter with GFP containing a degradation tag. Please view our [http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation#rhamnose Wiki] for more details about this component of our project.
This results were very promising. This graph indicates that not only can we induce induce expression rhamnose, but the promoter is quite tightly regulated with little leaky expression over a basal level. This makes it an excellent candidate for a killswitch construct, as it would allow for very little leaky expression of any kill genes when not wanted and an easy way of inducing the kill gene expression. We later tested this promoter out with an S7 micrococcal nuclease. This data can be found here
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 910
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