Generator

Part:BBa_K902002

Designed by: Emily Hicks   Group: iGEM12_Calgary   (2012-08-29)
Revision as of 21:48, 26 October 2012 by Rpgguardian (Talk | contribs)

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Beta-glucuronidase (uidA) inducible generator

This part contains the beta-glucuronidase (uidA) enzyme from E. coli under the control of an IPTG inducible promoter.

Usage and Biology

The uidA gene encodes for the <beta>-D-glucuronidase enzyme most commonly known for its use in the Gus assay. It is also known as the gusA gene and has numerous homologous in various bacterial and plant species. It has specific activity against glucuronoside linked to a variety of other components. This is commonly used with substrates such as X-Gluc, or PNPG (p-nitrophenol-glucuronoside) which is the substrate our team has used to characterize its activity electrochemically. See our experience page for more information on its characterization.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 732
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None