Part:BBa_K801063:Design

(+)-limonene synthase 1 expression cassette for yeast

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 2127
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 211
  Illegal SapI.rc site found at 561
  

This is a composite part consisting of the yeast TEF1 promoter (BBa_K319003), the Citrus limon (+)-limonene synthase 1 coding region (BBa_K801061) and the yeast TEF1 terminator (BBa_K801011).

Keywords: limonen

Abbreviations:

Design Notes

Related BioBrick:

• BBa_K801060: (+)-Limonene synthase 1 with Strep-tag and yeast consensus sequence
• BBa_K801061: (+)-Limonene synthase 1 coding region
• BBa_K801062: (+)-limonene synthase 1 expression cassette for yeast
• BBa_K801064: (+)-limonene synthase 1 expression cassette for yeast
• BBa_K801065: (+)-limonene synthase 1 with Strep-Tag
• BBa_K801066: (+)-limonene synthase 1 with Yeast Consensus Sequence for improved expression

Quality control measures:

Backbone:

• Backbone name: pSB1C3
• Resistance: Cp
• Copynumber: high

Protein coding:

• BioBrick encodes (+)-limonene synthase
• UniProt entry: [http://www.uniprot.org/uniprot/Q8L5K3 Q8L5K3]

Enzymatic activity:

• E.C. Number: [http://enzyme.expasy.org/EC/4.2.3.20 4.2.3.20]

Cytotoxicity:
cytotoxic for S.cervisiae growth inhibition at 1 mM, lethal effect at 100 mM

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:

• BioBrick: BBa_I742111

Organism:

• Gene sequence derived from Citrus limon

References

Literature references:

• http://www.ncbi.nlm.nih.gov/pubmed/12084056 Lücker et al., 2002 Lücker, J., El Tamer, M. K., Schwab, W., Verstappen, F. W. A., van der Plas, L. H. W., Bouwmeester, H. J., and Verhoeven, H. A. (2002). Monoterpene biosynthesis in lemon (Citrus limon). cDNA isolation and functional analysis of four monoterpene synthases. Eur J Biochem, 269(13):3160–71.

Database references: