Coding
BAMT
Part:BBa_J45002:Design
Designed by: André Green II Group: iGEM06_MIT (2006-06-07)
SAM:benzoic acid carboxyl methyltransferase; converts benzoic acid to methyl benzoate (floral odor)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 970
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 654
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
- There are two Met codons at the N-terminus of the protein (putative codons 1 and 4). It is believed that the first Met codon is the actual start of the protein. Reference:
<biblio>
- Dudareva pmid=11051108
</biblio>
- Forward primer (5' -> 3'): GTT TCT TCG AAT TCG CGG CCG CTT CTA G ATG AAA GTG ATG AAG AAA C
- Bases 1-18 of the original A. majus sequence (gccggacgccaaagaaaa) were deleted. Thus, this sequence begins with the first start codon.
- A silent mutation (A -> G) was made at base 702 to eliminate an SpeI cut site.
- The original stop codon (TGA at bp 1093) was deleted and a double TAA stop was inserted
Source
Genbank accession number(s): AF198492
We got this cDNA from N Dudareva at Purdue.