Reporter
Part:BBa_K900000:Design
Designed by: Austin Jones Group: iGEM12_Berkeley (2012-09-27)
Revision as of 01:39, 4 October 2012 by Robertchen1992 (Talk | contribs)
PAmCherry (photoactivatable red fluorescent protein)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 682
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The gene was codon optimized for yeast and internal restriction sites were removed for compatibility with our Golden Gate cloning scheme.
Source
Synthesized in house. Previously engineered version of RFP from Subach et al., 2009.
References
Subach, F. V. et al. Photoactivatable mCherry for high-resolution two-color fluorescence microscopy. Nature Methods 6, 153–159 (2009).