Generator
Part:BBa_K902048:Experience
Designed by: Emily Hicks, Stephanie Willatt Group: iGEM12_Calgary (2012-10-02)
Revision as of 01:13, 4 October 2012 by Stephanie0101 (Talk | contribs)
XylE (J33204) was biobricked under the tetR promoter (R0040). This construct was confirmed by sequencing and used in visual assays. The visual assay shown below demonstrated that the XylE gene was expressed with the tetR promoter and that the resulting enzyme (catechol 2,3-dioxygenase) was functional. The colour change to bright yellow indicated the conversion of catechol to 2-hydroxymuconic semialdehyde.
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