Coding

Part:BBa_K842006

Designed by: Sean P. Curran, Percy Genyk, Ellen Park, Rebecca, Gao, Stephan Genyk, Megan Bernstein, Rachel Kohan, Eric Siryj, Luke Quinto   Group: iGEM12_USC   (2012-10-02)
Revision as of 01:12, 4 October 2012 by Rkohan (Talk | contribs)

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cheA

USC chea.gif

CheA is a regulatory protein that becomes activated when the methyl-accepting chemotaxis protein (MCP) is not bound to any attractant. The activated cheA self-phosphorylates itself by hydrolyzing ATP in order to gain a phosphate group. CheA then donates its phosphate group to activate cheB or cheA.

Used for researcher control of signaling mechanisms that influence flagella function.

Cloning and Uses This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. It is to be used with other chemotaxis genes to form a complete regulatory pathway that controls the response system in MCP and the direction of rotation in flagella.














Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1286
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1286
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1286
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1286
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1286
    Illegal NgoMIV site found at 1636
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None