Part:BBa_K842003
fliA
fliA is an alternate sigma factor for the class 3 flagella operons. When transcribed, it forms a component of RNA polymerase sigma 28.
Used for reconstitution of flagella apparatus in E. coli B strains.
32 and 28 belong to the 3rd group of Sigma factors. Sigma factors of that group contain of 2, 3 and 4 areas and structurally are really diverse. 28 is involved in the synthesis of flagell in E.coli(Paget, 2015). For that work of sigma factor the great value has a weaker capacity to the promoter melting. It has been shown that having the strict consensus in the promoter region is important for 28 , it provides a strict specifity of recognition in the initiation of transcription(Koo et al, 2009).
Shown consensus:
It may be explained with that fact, that this type of sigma factors may not have one or several amino acid residues, required for villiany -11A when you initiate promoter region melting(Feklístov et al, 2014).
Pic. 1. Alignment of amino acid sequences of the alternative Sigma relative to the main one. Area 3. 2 is shown in red.
Cloning and Uses:
This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. It is to be used in conjunction with the entire flagella synthesis genetic pathway to reproduce a complete flagella apparatus.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3
Illegal PstI site found at 91 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3
Illegal PstI site found at 91 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 3
Illegal PstI site found at 91 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 3
Illegal PstI site found at 91 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 426
Illegal SapI.rc site found at 451
Illegal SapI.rc site found at 607
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