Part:BBa_K847002:Experience

Assay
Liquid cultures of NEB5α E. coli transformed with MntH, recA, sod Cu/Zn, Dps 1 (Sod Mn and Dps 2 constructs could not be completely assembled in time), and negative control were grown up over night at 37°C. The following day, the cells were washed and resuspended in 0.9% NaCl solution. Cell concentration was then adjusted to 10^7/mL in 5mL of a glass Petri dish. Each sample was then exposed to 1.2 J/(m^2*sec) of UV-C radiation from a UV lamp for a cumulative of 0 seconds, 2 seconds, 5 seconds, 10 seconds, 20 seconds, and 30 seconds. After each exposure, a dilution spot assay was conducted to determine the final number of surviving cells.

Results
Characterized; however, further testing needed. Refer to http://2012.igem.org/Team:Stanford-Brown/HellCell/Radiation.

Applications of BBa_K847002

Astrobiology revolves around three central questions: "Where do we come from?", "Where are we going?", and "Are we alone?" To approach the second question, the Hell Cell subgroup of the Stanford-Brown iGEM team developed BioBricks that allow a cell to survive harsh extraterrestrial conditions. Such a toolset could create a space-ready synthetic organism to perform useful functions off-world. This gene is one of the toolset, potentially conferring radiation resistance to otherwise radiation-intolerant bacteria.

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