Composite
aldA

Part:BBa_K892010

Designed by: David Zong, Felix Ekness   Group: iGEM12_Washington   (2012-09-30)
Revision as of 00:34, 4 October 2012 by Davidzng (Talk | contribs) (→‎Usage and Biology)

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aldA

The gene aldA, which codes for glycolaldehyde dehydrogenase, is one of the genes required for E. coli to utilize ethylene glycol as a food source. The coding sequence is put behind a strong biofab promoter and RBS. This part should be used with fucO for the desired effect.

Usage and Biology

This part was characterized by [http://2012.igem.org/Team:Washington Washington 2012] using the turbidostat. MG1655 cells were cotransformed with fucO and aldA, the assay is described on the [http://2012.igem.org/Team:Washington/Protocols/EG_Assay wiki] and the data is also presented on the [http://2012.igem.org/Team:Washington/Plastics#Results wiki].

The fucO aldA protein pathway as part of the plastic degradation project

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 779
    Illegal PstI site found at 1492
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 779
    Illegal PstI site found at 1492
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 317
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 779
    Illegal PstI site found at 1492
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 779
    Illegal PstI site found at 1492
    Illegal AgeI site found at 589
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aaldA