Composite
fucO

Part:BBa_K892009

Designed by: David Zong, Felix Ekness   Group: iGEM12_Washington   (2012-09-30)
Revision as of 23:40, 3 October 2012 by Davidzng (Talk | contribs) (→‎Usage and Biology)

fucO

The gene fucO, which codes for glycolaldehyde reductase, is one of the genes required for E. coli to utilize ethylene glycol as a food source. The coding sequence is put behind a strong biofab promoter and RBS. This part should be used with aldA for the desired effect.

Usage and Biology

This part was characterized by [http://2012.igem.org/Team:Washington Washington 2012] using the turbidostat. MG1655 cells were cotransformed with fucO and aldA, the assay is described on the [http://2012.igem.org/Team:Washington/Protocols/EG_Assay wiki] and the data is also presented on the [http://2012.igem.org/Team:Washington/Plastics#Results wiki].


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 251
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 251
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 251
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 251
    Illegal AgeI site found at 967
    Illegal AgeI site found at 1168
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/afucO