Coding
recA

Part:BBa_K847000:Design

Designed by: Rashmi Sharma, Vishesh Jain, Kendrick Wang   Group: iGEM12_Stanford-Brown   (2012-08-09)
Revision as of 00:24, 4 October 2012 by Rsharma (Talk | contribs) (→‎Design Notes)

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''Deinococcus radiodurans'' DNA repair mechanism, Recombinase A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 224
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 898
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was isolated via colony PCR from Deinococcus radiodurans. It does not contain promoter, RBS, transcriptional terminator, etc. These elements must be connected to this part for proper functionality.

Source

Deinococcus radiodurans

References

Daly, M.J. (2009) A new perspective on radiation resistance based on Deinococcus radiodurans. Nature Rev. Microbiol, 7: 237-245.

Slade, D. and Miroslav, R. (2011). Oxidative stress resistance in Deinococcus radiodurans. Microbiol. Mol. Biol. Rev., 75(1): 133-191.