Composite

Part:BBa_K801077:Design

Designed by: Dennis Hell   Group: iGEM12_TU_Munich   (2012-09-24)
Revision as of 09:47, 23 October 2012 by Dennis48 (Talk | contribs) (→‎Design Notes)

Caffeine Synthesis Pathway pTEF2-XMT1-tADH1-pTEF1-MXMT1-tADH1-pTEF2-DXMT1-tADH1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3613
    Illegal BglII site found at 5829
    Illegal BglII site found at 5925
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 138
    Illegal NgoMIV site found at 4317
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2385


Composite part of BBa_K801010 BBa_K801070 BBa_K801012 BBa_K319003 BBa_K801071 BBa_K801012 BBa_K801010 BBa_K801072 BBa_K801012


Keywords: caffeine, TEF1,


Abbreviations:

Design Notes

Related BioBrick:

Quality control measures:

Backbone:

  • Backbone name: pSB1C3'

Protein coding:

Enzymatic activity:

Cytotoxicity:
This Biobrick includes all enzymes of the pathway which leads to caffeine. We investigated the toxicity of caffeine and found out, that the growth of yeast strains was inhibited at concentrations of 10 mM. Caffeine with a concentration of 100 mM even showed a lethal effect on yeast cells. Lower concentrations of caffeine (range of micro molar) did not show any growth inhibition or effect.

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:


Organism:


References

Literature references:

Database references: