Part:BBa_K801063:Design

(+)-limonene synthase 1 expression cassette for yeast

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 2127
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 211
  Illegal SapI.rc site found at 561
  

This is a composite part consisting of the yeast TEF1 promoter (BBa_K319003), the Citrus limon (+)-limonene synthase 1 coding region (BBa_K801061) and the yeast TEF1 terminator (BBa_K801011).

Keywords: limonen

Abbreviations:

Design Notes

Related BioBrick:

• BBa_K801060: (+)-Limonene synthase 1 with Strep-tag and yeast consensus sequence
• BBa_K801061: (+)-Limonene synthase 1 coding region
• BBa_K801062: (+)-limonene synthase 1 expression cassette for yeast
• BBa_K801064: (+)-limonene synthase 1 expression cassette for yeast
• BBa_K801065: (+)-limonene synthase 1 with Strep-Tag
• BBa_K801066: (+)-limonene synthase 1 with Yeast Consensus Sequence for improved expression

Quality control measures:

Backbone:

• Backbone name: pSB1C3'

Protein coding:

Enzymatic activity: EC-number 4.2.3.20

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:

Organism:

• Genesequence derived from citrus limon

References

Literature references:

Database references: