Part:BBa_K801091:Design

phenylalanine ammonia lyase (PAL) coding region

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2090
  Illegal BamHI site found at 352
  Illegal XhoI site found at 403
  Illegal XhoI site found at 466
  Illegal XhoI site found at 484
  Illegal XhoI site found at 562
  Illegal XhoI site found at 763
  Illegal XhoI site found at 1006
  Illegal XhoI site found at 1753
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 922
  Illegal NgoMIV site found at 1168
  Illegal NgoMIV site found at 1336
  Illegal NgoMIV site found at 1479
  Illegal NgoMIV site found at 1813
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 463
  Illegal BsaI site found at 793
  Illegal BsaI site found at 799
  Illegal BsaI.rc site found at 1924
  Illegal SapI site found at 753
  

Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA

http://www.ncbi.nlm.nih.gov/nuccore/AX366866

PMID: 15932991

Keywords: Coumaryol-CoA, Coumaroyl-Coenzyme A, Xanthohumol, Biosynthesis, 4-coumarate-coenzyme A ligase

Abbreviations: PAL = Phenylalanine Ammonia Lyase

Design Notes

Related BioBrick:

Other versions: BBa_K801090: PAL + yeast consensus sequence

Related BioBricks:

• BBa_K801090: PAL + yeast consensus sequence
• BBa_K801092: 4-coumarate - coenzym A ligase (4CL) + yeast consensus sequence
• BBa_K801093: 4-coumarate--coenzyme A ligase (4CL) coding region
• BBa_K801094: Naringenin - chalcone synthase (CHS) + yeast consensus sequence
• BBa_K801095: Naringenin - chalcone synthase (CHS) coding region
• BBa_K801096: Aromatic prenyltransferase (APT) coding region
• BBa_K801097: Chalcone O-methyltransferase (OMT1) + yeast consensus sequence
• BBa_K801098: O-methyltransferase 1 (OMT1) coding region

Cloning details:
Designed in RFC10

Quality control measures:
Part was totally sequenced

Backbone:

• Backbone name: pSB1C3
• Resistance: Cp
• Copynumber: high

Protein coding:

• Protein: Phenylalanine Ammonia Lyase
• Tag: c-terminally fused Strep

Enzymatic activity: EC-number 4.3.1.25

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:
Plasmid: pKS2µHyg-PAL-4CL-CHS, provided by John A. Morgan, Purdue University, USA

Organism:
Genesequence derived from Rhodosporidium toruloides'

References

Literature references:
[http://www.ncbi.nlm.nih.gov/pubmed/15932991 Pubmed : Jiang, H., Wood, K.V., Morgan, J.A., 2005. Metabolic Engineering of the Phenylpropanoid Pathway in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 71, 2962–2969]

Database references:
[http://www.ncbi.nlm.nih.gov/nuccore/AX366866 GenBank: Sequence 18 from Patent WO0208402]

[http://www.uniprot.org/uniprot/P11544 Uniprot: Phenylalanine/tyrosine ammonia-lyase]