![](https://parts.igem.org/images/partbypart/icon_regulatory.png)
Regulatory
polAP
Part:BBa_K847210
Designed by: Bryce Bajar, Jason Hu, Chris Jackson, Debha Amatya, Ben Geilich Group: iGEM12_Stanford-Brown (2012-07-24)
polA (DNA Polymerase I) DnaA-activated promoter
The polA promoter (polAP) is a DNA-replication dependent promoter.
Usage and Biology
DnaA is the central initiator of DNA replication in E. coli and other prokaryotic organisms, but importantly, it also functions as a transcription factor that can suppress or activate transcription of genes by binding to the DnaA box, a 9bp consensus sequence (Messer 1997). Since DnaA expression is dependent on the growth conditions of the cell (Chiaramello 1990), genes regulated by DnaA are transitively growth dependent. One such DnaA-dependent gene is polA, which codes for DNA Polymerase I, active in DNA replication (Quiñones 1997).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 144
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
//promoter
//promoter
biology | |
control |