DNA

Part:BBa_K323066:Experience

Designed by: Jernej Turnšek   Group: iGEM10_Slovenia   (2010-10-20)
Revision as of 05:10, 3 October 2012 by Ez810711 (Talk | contribs)


We used this part in combination with parts BBa_K323132 and BBa_K323135 and showed that this DNA program leads to higher yields of violacein production. Check results.

Applications of BBa_K323066

Experience by 2012 iGEM NCTU_FORMOSA

The design of our biobricks(Part:BBa_K887000) has the same order of zinc finger as 2010 Slovenia iGEM team, so we decided to use the DNA program (Part:BBa_K323066)

However, after sequencing, we found that there is a deletion of a base pair in the zif268 biding site.

DDpicture1.jpg


So, we designed a complementary primer to insert the lost base pair. After PCR of point mutation, we sequence it again.

And the outcome is below

DDpicture2.jpg


As a result, we successfully correct the lost G base at the zif268 binding site.

User Reviews

UNIQ55937d02b2e683aa-partinfo-00000000-QINU UNIQ55937d02b2e683aa-partinfo-00000001-QINU