Terminator

Part:BBa_K799029

Designed by: Daniel Wolozny   Group: iGEM12_Johns_Hopkins-Wetware   (2012-09-25)
Revision as of 02:14, 3 October 2012 by Danielw9 (Talk | contribs)

MFA2 Terminator

Terminator of the MFA2 gene from Saccharomyces Cerevisiae. It confers relative instability to the mRNA produced, increasing the amount of mRNA made and protein produced.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 217

Plasmid Map

MFA2t plasmid.png

Sequencing

MFA2t sequence.png

This is the sequencing result for the MFA2 gene terminator. As can be seen, it is a perfect match to the genomic DNA terminator.

[edit]
Categories
Parameters
None