Coding
Part:BBa_K804010:Experience
Designed by: Joe Ho Group: iGEM12_British_Columbia (2012-10-02)
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K804010
User Reviews
UNIQ043e971c27ada184-partinfo-00000000-QINU UNIQ043e971c27ada184-partinfo-00000001-QINU
This part consists of an arabinose promoter, a strong RBS, and the metA coding gene. It is able to complement a metA knockout, and the growth rate appears to be proportional to the amount of arabinose that is added.
To generate this graph, we cultured the metA auxotroph transformed with this part in M9 media and added the described amount of arabinose. The OD600 of the cultures were measured every 15 minutes, and was run overnight.