Composite

Part:BBa_K824008:Experience

Designed by: Steven Allen   Group: iGEM12_Gaston_Day_School   (2012-10-01)
Revision as of 11:47, 17 October 2014 by Annebyford (Talk | contribs) (User Reviews)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K824008

User Reviews

UNIQ015f78349a70d1c6-partinfo-00000000-QINU UNIQ015f78349a70d1c6-partinfo-00000001-QINU Oct. 2014: Part K1320000 contains the same cadmium responsive promoter and GFP but includes sensitivity tuners. It is far more sensitive than this part (2.5mM Cd).

We grew liquid cultures for 24 hours with varying concentrations of Cadmium Nitrate in the presence of our cells. After growth, the tests were spun down, then resuspended in 1X PBS (Phosphate Buffer Solution). The results were measured in fluorescence per OD 600. The graph below shows our results.


Cadgraphuse.jpg