Composite

Part:BBa_K887004

Designed by: WU, CHENG-HSUAN   Group: iGEM12_NCTU_Formosa   (2012-09-21)
Revision as of 11:20, 2 October 2012 by Max07 (Talk | contribs)

Plac+alsS+ilvC+ilvD(each preceded by own zinc-finger and RBS)+Ptet+B0032+kivD+B0015


Picture13.jpg
This part（BBa_87004) ,which is ligated from Biobrick(Part:BBa_K887000) with another one(Part:BBa_K539741)contain four enzymes. Each of the former three enzymes(alsS, ilvC& ilvD) linked with a zinc finger. The three enzymes could convert pyruvate into 2-ketoisoalerate(the Intermediate of isobutanol). The final enzyme(kivD)could change 2-ketoisoalerate into isobutyraldehyde, which would be converted into isobutanol by ADH in E.coli. This is a complete circuit to produce isobutanol.


The main goal of our project this year is to improve the production of isobutanol in E.coli. In addition to the temperature control system, we also find the zinc fingers to be a perfect solution for our project. According to 2010 Slovenia team, the yield of violacein was at least 2-fold higher with the application of the zinc finger. Therefore, We expect higher concentration of isobutanol.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 7718
    Illegal XhoI site found at 6049
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6938
    Illegal AgeI site found at 3158
    Illegal AgeI site found at 4143
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2744
    Illegal BsaI site found at 5835
    Illegal BsaI site found at 6092
    Illegal BsaI.rc site found at 836
    Illegal BsaI.rc site found at 1430
    Illegal BsaI.rc site found at 3563


[edit]
Categories
Parameters
n/aPlac+alsS+ilvC+ilvD(each preceded by own zinc-finger and RBS)+Ptet+B0032+kivD+B0015