Part:BBa_K847080:Experience

Assay
Liquid cultures of negative control, recA, dps, and sdaB transformed NEB5α E. coli were grown up over night at 37°C. After incubation, LB + amp media of pH 3.54, 4.60, 6.93, 9.07, 9.50 were created using HCl and NaOH. In a 96 well plate, 8 wells were filled with LB + amp blank. Next, 5uL of each transformed bacteria was placed in five different pH wells. This was replicated four times, thus, creating a 96 well plate with 8 blanks, and four replicates of each test construct at five different pH’s. Finally, the OD600 was measured every five minutes for 24 hours while the plate was incubated at 37°C, shook for 1 minute before every sample and between every sample in a spectrophotometer.

Results
Works. Refer to: http://2012.igem.org/Team:Stanford-Brown/HellCell/pH

User Reviews

UNIQ4448a20a317500a6-partinfo-00000000-QINU UNIQ4448a20a317500a6-partinfo-00000001-QINU