T7
Part:BBa_K921000
Designed by: Eric Pederson Group: iGEM12_Carnegie_Mellon (2012-10-01)
Revision as of 01:43, 2 October 2012 by Enpederson (Talk | contribs)
T7 RNAP + IPTG->PoPs (Mutant I)
This is a mutant T7 promoter that includes a lacO sequence. This is repressible by the LacI protein and is induced in the presence of IPTG. The promoter sits in the 5' region of the gene of interest and initiates transcription when the cell expresses T7 RNA polymerase. Be sure you are using a cell strain that is compatible with T7 expression vectors.
The following parameters were calculated and compared against the "wild-type" [1] The following parameters were calculated:
Promoter | BBa K921000 | BBa K921001 | BBa K921002 |
---|---|---|---|
Transcription Strength | 97% | 72% | 127% |
Translational Efficiency | 6% | 6% | 94% |
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
direction | Forward |
efficiency | 169% |
negative_regulators | LacI (Hypothesized LacIQ compatible) |
[edit]
Categories
Parameters
//chassis/bacteriophage/t7
//function/regulation/transcriptional
//plasmid/expression/t7
//promoter
//regulation/negative
//function/regulation/transcriptional
//plasmid/expression/t7
//promoter
//regulation/negative
direction | Forward |
efficiency | 169% |
negative_regulators | LacI (Hypothesized LacIQ compatible) |