Coding
ATF1

Part:BBa_J45006

Designed by: Veena Venkatachalam   Group: iGEM06_MIT   (2006-06-07)
Revision as of 21:48, 7 June 2006 by Veenav (Talk | contribs) (→‎Usage and Biology)

alcohol acetyltransferase I (ATF1)

From S. cerevisiae (yeast)

Usage and Biology

  • The gene ATF1 encodes the enzyme alcohol acetyltransferase 1, which produces acetate esters from acetyl CoA and alcohols. We will be interested in using ATF1 to catalyze the reaction of isoamyl alcohol -> isoamyl acetate; the product is of interest because it is a volatile ester with a strong banana-like scent.
  • Horton et al. reported a yield of 1.8mM isoamyl acetate 40 hours after the addition of 10mM isoamyl alcohol to cultured E. coli expressing the ATF1 gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 99
    Illegal BamHI site found at 1426
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 526
    Illegal SapI.rc site found at 1534


[edit]
Categories
//function/odor
Parameters
None