Coding
dszA
Part:BBa_K902050:Design
Designed by: Lisa Oberding, Ali Honarmand Group: iGEM12_Calgary (2012-10-01)
Revision as of 02:44, 4 October 2012 by Jeffaddison89 (Talk | contribs)
DBT-sulfone monooxygenase (DszA) from R. erythropolis
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 754
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 43
Illegal NgoMIV site found at 258
Illegal NgoMIV site found at 562
Illegal NgoMIV site found at 682
Illegal AgeI site found at 217 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1066
Design Notes
4 internal PstI cut-sites had to be mutated with site-directed mutagenesis.
Source
Obtained from PCR amplification from a plasmid found in an environmental isolate of R. erythropolis shown to be an active desulfurizer.
References
Gou,Z., Li,X., Xing,J., Liu,H. and Chen,J. LSSE8-1 and its desulfurizational genes. Laboratory of Separation Science and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 1# Beiertiao, Zhongguancun, Haidian, Beijing 100080, China. 2003.