Generator

Part:BBa_K741003

Designed by: Wuyang Chen   Group: iGEM12_USTC-China   (2012-07-24)
Revision as of 08:03, 30 September 2012 by Dunkle (Talk | contribs) (zs)

pRM-RBS-GFP-T

Express GFP only if protein CI activated the promoter pRM.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 752

USTC2012Result2.png

Figure 2 shows that the fluorescence intensity of prm-gfp is so weak in comparison with plac-gfp that we can assume that the leak expression of prm is very few, which doesn't affect our experiments.

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Categories
Parameters
None