Generator

Part:BBa_K772005

Designed by: Mustafa Elitok   Group: iGEM12_Fatih-Medical   (2012-09-25)
Revision as of 11:09, 29 September 2012 by Mustafa (Talk | contribs)

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AHL generator: LasI

Quorum sensing is the mechanism of communication between bacteria via synthesizing and detecting chemical compounds in the media. With these mediators, the promoters and their yield can be tuned or regulated. This part represents an alternative quorum sensing sender device which is designed to induce PoPS production in the parts including pLas such as BBa_K772004[1]. LasI is an enzyme generator gene sequences which synthesizes 3OC12HSL (AHL) in order to induce a PoPS response in the pLas promoter. pLas promoter works in the presence of both 3OC12HSL and LasR protein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1581
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/transcriptionalregulator
//chassis/prokaryote/ecoli
//classic/regulatory/other
//classic/signalling/sender
//function/regulation/transcriptional
regulator
Parameters
chassisE.coli TOP10 strain
device_typeRegulatory/Sender
functionQuorum sensing
input_sIPTG
negative_regulatorsLacI
output_s3OC12HSL
positive_regulatorsIPTG
signalling_molecule3OC12HSL