Part:BBa_K771002
Membrabe Anchor 2:ssDsbA-Lgt-SH3 Domain-MS2
Membrane anchor 2:RNA aptamer binding protein MS2 (BBa_K771112) fused with membrane protein Lgt(BBa_K771102).
Overview: Membrane Scaffold System
Demonstration of the whole Membrane Scaffold device. Membrane Anchor 2, 3, 4, 5 consitutively aggregate while Membrane Anchor 1 and 6 only aggregate with constitutive cluster (Membrane Anchor 2, 3, 4, 5) when certain signal is present.
Backgroud
Signal Induced Aggregation
Membrane Anchor 1 and 2 act as a controlling point to determine whether certain pathway (in this case involving enzyme 1, 2, 3, 4 and 5) is activated or not. RNA molecule D0(BBa_K771009) with PP7 and MS2 aptamer domain acts as the signal.
Constitutive Aggregation
Membrane Anchor 2 and 3 constitutively aggregate through SH3 domain(BBa_K771107) and SH3 ligand (BBa_K771108).
Design
RNA aptamer binding protein MS2(BBa_K771112) fused with membrane protein Lgt (BBa_K771102). When RNA molecule D0 is present, Membrane Anchor 1(BBa_K771001) is expected to dimerize with Membrane Anchor 2 through MS2(BBa_K771112) and PP7(BBa_K771111) domain.
Related Biobrick
<br\>Membrabe Anchor 1([BBa_K771001]) <br\>Membrabe Anchor 3([BBa_K771003]) <br\>Membrabe Anchor 4([BBa_K771004]) <br\>Membrabe Anchor 5([BBa_K771005]) <br\>Membrabe Anchor 6([BBa_K771006])
Reference
Delebecque, C. J., A. B. Lindner, et al. (2011). "Organization of intracellular reactions with rationally designed RNA assemblies." Science 333(6041): 470.
Dueber, J. E., G. C. Wu, et al. (2009). "Synthetic protein scaffolds provide modular control over metabolic flux." Nature biotechnology 27(8): 753-759.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 883
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 504
Illegal BsaI site found at 1609
Illegal SapI.rc site found at 1150
n/a | Membrabe Anchor 2:ssDsbA-Lgt-SH3 Domain-MS2 |