Reporter
Part:BBa_K900000:Design
Designed by: Austin Jones Group: iGEM12_Berkeley (2012-09-27)
Revision as of 22:29, 3 October 2012 by Austin.jones (Talk | contribs)
Design Notes
The gene was codon optimized for yeast and internal restriction sites were removed for to compatibility with our Golden Gate cloning scheme.
Source
Synthesized in house and cloned into addgene plasmid [http://www.addgene.org/11911/ l11911] Previously engineered version of RFP from Subach et al. (non-genomic DNA sequence).
References
Subach, F. V. et al. Photoactivatable mCherry for high-resolution two-color fluorescence microscopy. Nature Methods 6, 153–159 (2009).