File:T7rsr graph 3.png

Our biobrick is designed to be an integration of two parts, the protein part and the R-S-R part. Sequences of proteins Cas3 and cascade complex subunits are located in the upstream of the RSR system.

The number of the repeat and spacer are not fixed and the distance from the promoter does not affect the functionality.

The protein part in the biobrick is not essential for the system to work, as the type I-E CRISPR/Cas system is an endogenous system in E. coli. Since this part is designed to improve the sufficiency of system functionality, we choose to use Pveg, a strong constitutive promoter. This choice also makes it possible to utilize this system in other microorganisms where CRISPR system does not exist naturally, such as Bacillus subtilis.