Device

Part:BBa_K771003

Designed by: Guo Huaqing   Group: iGEM12_SJTU-BioX-Shanghai   (2012-09-25)
Revision as of 06:30, 28 September 2012 by AleAlejandro (Talk | contribs) (→‎Membrane Accelerator System)

Membrane Anchor 3 :ssDsbA-PDZ Ligand-LGT-SH3 Ligand

Membrane anchor 3, which consists of interacting SH3 domain and membrane protein Lgt. Membrane Anchor 3 is proved to constitutively dimerize with Membrane Anchor 2 [BBa_K771002].


Membrane Accelerator System

Membrane accelerator.jpg

All four membrane proteins are assembled together through interacting domains and ligands. Downstream enzymes are thus assembled.

It has been proved to work as expected by recruiting enzymes involved in Fatty Acid biosynthetic pathway.

Membrane Rudder System

12SJTU-MA1-MA6.jpg

Demonstration of the whole Membrane Rudder device.


Related Biobrick

<br\>Membrabe Anchor 1([BBa_K771001]) <br\>Membrabe Anchor 2([BBa_K771002]) <br\>Membrabe Anchor 4([BBa_K771004]) <br\>Membrabe Anchor 5([BBa_K771005]) <br\>Membrabe Anchor 6([BBa_K771006])

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 904
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 525


[edit]
Categories
Parameters
n/aMembrane Anchor 3 :ssDsbA-PDZ Ligand-LGT-SH3 Ligand