Part:BBa_K771002

Membrabe Anchor 2:ssDsbA-Lgt-SH3 Domain-MS2

Membrane Anchor 2:RNA aptamer binding protein MS2 fused with membrane protein Lgt. And it is part of Membrane Rudder system.

Membrane Rudder System

Demonstration of the whole Membrane Rudder device. When RNA signal molecule is present, Membrane Anchor 1 and 2 dimerize, so enzyme 1, 2, 3 ,4 and 5 aggregate to activate one of the pathways.

RNA Rudder

Membrane Anchor 1 and 2 act as a controlling point to determine whether certain pathway （in this case involving enzyme 1, 2, 3, 4 and 5) is activated or not.

A: Sketch of signal RNA molecule (called D0) which consists of PP7 and MS2 aptamer domains. B: Through fusing PP7 and MS2 protein to our membrane device, RNA molecule in Figure A can function as a bridge to connect different proteins, thus decreasing distance between corresponding enzymes.

Related Biobrick

<br\>Membrabe Anchor 2([BBa_K771002]) <br\>Membrabe Anchor 3([BBa_K771003]) <br\>Membrabe Anchor 4([BBa_K771004]) <br\>Membrabe Anchor 5([BBa_K771005]) <br\>Membrabe Anchor 6([BBa_K771006])

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 883
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 504
  Illegal BsaI site found at 1609
  Illegal SapI.rc site found at 1150