Regulatory
PfadR

Part:BBa_K861060:Experience

Designed by: Kuanwei Sheng   Group: iGEM12_WHU-China   (2012-09-08)
Revision as of 03:55, 30 October 2012 by Shinkwee (Talk | contribs)

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Applications of BBa_K861060

Result

Normalized using Fluorescence/0D600

Green: PfadR

Glucose Concentration gradient: 1, 10 mM

Fatty acid Concentration gradient: 0.025, 0.05, 0.1, 0.5,1.5 mM

PfadR promoter strength at different glucose and fatty acids concentration

As can be shown from the results, the promoter shows about 3 times induction from glucose to 1.5umol/L fatty acids medium and the fluorescence of PfadR. Also, in medium that use glucose as sole carbon source, PfadR seems to be leaky. However, since our bacteria is wild type E.coli, Fab genes was not mutated, meaning that bacteria can synthesis fatty acids. Therefore, there may be a basal level fatty acids concentration inside the cell, making the transcription not being totally repressed.

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