Composite

Part:BBa_K953000

Designed by: Ryan Kenny   Group: iGEM12_Macquarie_Australia   (2012-09-20)
Revision as of 02:00, 27 September 2012 by DanWinter (Talk | contribs)

Heme Oxygenase with T7 & RBS (E. coli Codon Optimised)

Synechocystis Heme Oxygenase with a RBS and codon optimised for expression in E. coli. This part differs from Part BBa_K953004 in that it also includes a T7 promoter (Part BBa_I712074). Heme oxygenase will oxidise heme into biliverdin and can then complex with bacteriophytochrome (Part BBa_K953001 or Part BBa_K953002). This complex can then absorb red light (620-750 nm) to excite the bacteriophytochrome and result in a phenotypic change from blue to green. This can be reversed by far-red light (700-800 nm) or will revert from green to blue over time. As E. coli does not produce biliverdin, heme oxygenase must be coupled with a bacteriophytochrome to activate the oxidation of heme to produce biliverdin.


http://25.media.tumblr.com/tumblr_mazgigGnrs1rg4kjpo1_500.jpg
Pellets of cells containing induced heme oxygenase, uninducded heme oxygenase and lacking heme oxygenase



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 314
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aHeme Oxygenase with T7 & RBS (E. coli Codon Optimised)