Generator

Part:BBa_K778005

Designed by: Yuka Yamazaki   Group: iGEM12_UT-Tokyo   (2012-09-23)
Revision as of 05:49, 6 October 2012 by YutaOtsuka (Talk | contribs)

RBS-fhlA363-d.term

FhlA-E363G generator without promoter (RBS-fhlAE363G-d.term)

FhlA is the trnascriptional activator of hycAp Part:BBa K778000 . E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)). fhlA-E363G has one point mutation (A to G, glutamic acid to glycine) from wild-type fhlA. This mutation increased H2 production under the conditions in the previous research (Viviana et.al.2009). Actually, in our research (team UT-Tokyo 2012), this mutation increased H2 production as the following figure. (The full construct in this experiment is Part:BBa K778008)

UT Tokyo H2 1001+eg.png

Detailed Information :Our wiki (UT-Tokyo 2012)[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]The results shown on the wiki is diffaerent from the latest result on this page.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2115
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1394
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None