Part:BBa_K864440

Small RNA spot42

The natural small RNA spot42 taken from the E-coli MG1655. Small RNA is belived to interact in metabolic pathways. The spot42 scaffold recruits Hfq proteins. In some cases it has been shown to bind to the ribosomal binding site of the mRNA and thereby blocking translation. ( V.Sharma, A.Yamamura Y.Yokobayashi, 2011 )

We wanted to use this sRNA for gene down regulation on translational level, in this case block the ribosome from binding to the rbs area.

You can use this natural scaffold to make your own sRNA that could inhibit translation of a gene in question. The sRNA is coupled to a strong promotor, J23101.

Reference

Engineering Artificial Small RNAs for Conditional Gene Silencing in Escherichia coli Vandana Sharma, Asami Yamamura, and Yohei Yokobayashi ACS Synthetic Biology 2012 1 (1), 6-13

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]