Terminator

Part:BBa_K801011:Design

Designed by: Georg Schtzinger   Group: iGEM12_TU_Munich   (2012-09-21)
Revision as of 22:45, 26 September 2012 by Giergl (Talk | contribs) (→‎Design Notes)

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TEF1 yeast terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Note: The submitted version of this part has a mutation in the BioBrick suffix: The NdeI restriction site has been lost. However, the part can still be used, because the SpeI and PstI restriction sites are functional.



Extracted from genomic DNA of S. cerevisiae using PCR.


Keywords:


Abbreviations:

Design Notes

Related BioBrick:

Quality control measures:
Test digestion using XbaI+PstI-HF, EcorI+ SpeI-HF

  • Sequencing using primer VR and VF2

Backbone:
Backbone name: pSB1C3

Protein coding:

Enzymatic activity:

Cytotoxicity:
none

Safety notes:
none

Intellectual property:

Corresponding part author/authors:

Source

Source:
PCR product from genomic DNA of Saccharomyces cerevisiae


Organism:
Saccharomyces cerevisiae

References

Literature references:

  • Sun, Jie; Shao, Zengyi; Zhao, Hua; Nair, Nikhil; Wen, Fei; Xu, Jian-He; Zhao, Huimin (2012): Cloning and characterization of a panel of constitutive promoters for applications in pathway engineering in Saccharomyces cerevisiae. In: Biotechnol. Bioeng. 109 (8), S. 2082–2092.
  • Guo, Z.; Sherman, F. (1996): 3'-end-forming signals of yeast mRNA. In: Trends Biochem. Sci. 21 (12), S. 477–481.


Database references: