Device

Part:BBa_K778011

Designed by: Yuka Yamazaki   Group: iGEM12_UT-Tokyo   (2012-09-23)
Revision as of 20:36, 26 September 2012 by YutaOtsuka (Talk | contribs)

hycAp-GFPgenerator-plac-FhlA363generator

You can "enhance" and "see" H2 production with this device.

hycAp-GFPgenerator-plac-FhlA363generator

GFP is a reportor of H2 production (strictly speaking,reporter of hycAp activation Part:BBa K778000 (hycAp is the promotor of formate hydrogen lyase of genome)).

FhlA is the trnascriptional activator of hycAp Part:BBa K778000 . E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)). fhlA-E363G has one point mutation (A to G, glutamic acid to glycine). Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3266
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2545
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 828


[edit]
Categories
Parameters
None